http://purl.uniprot.org/citations/9511743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9511743 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/9511743 | http://www.w3.org/2000/01/rdf-schema#comment | "The N-ethylmaleimide-sensitive fusion protein (NSF) is required for vesicular membrane fusion in multiple cellular functions. We have cloned a cDNA encoding the Dictyostelium discoideum homolog of the NSF protein. This cDNA hybridizes with a single fragment in Southern blots suggesting that NSF is encoded by a single gene in the amoeba. It is expressed constitutively during vegetative growth and throughout the differentiation cycle. The encoded gene product comprises 738 aa with a predicted molecular mass of 82 kDa. It shows the characteristic three-domain structure of NSF proteins. A more divergent amino-terminal part is followed by two highly conserved ATP-binding domains featuring Walker A and B signature sequences. The D. discoideum protein presents an overall aa sequence identity of 44% when compared to known NSF homologs. The monoclonal antibody 2E5 directed against Cricetellus griseus NSF recognizes a protein with a molecular weight of approx. 80 000 in a D. discoideum crude extract and the recombinant D. discoideum His6-NSF expressed in Escherichia coli."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(97)00604-5"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0378-1119(97)00604-5"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/author | "Satre M."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/author | "Satre M."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/author | "Bruckert F."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/author | "Bruckert F."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/author | "Weidenhaupt M."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/author | "Weidenhaupt M."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/name | "Gene"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/pages | "53-60"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/pages | "53-60"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/title | "Identification of the Dictyostelium discoideum homolog of the N-ethylmaleimide-sensitive fusion protein."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/title | "Identification of the Dictyostelium discoideum homolog of the N-ethylmaleimide-sensitive fusion protein."xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/volume | "207"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://purl.uniprot.org/core/volume | "207"xsd:string |
http://purl.uniprot.org/citations/9511743 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9511743 |
http://purl.uniprot.org/citations/9511743 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9511743 |
http://purl.uniprot.org/citations/9511743 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9511743 |
http://purl.uniprot.org/citations/9511743 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9511743 |