| http://purl.uniprot.org/citations/9512477 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9512477 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9512477 | http://www.w3.org/2000/01/rdf-schema#comment | "Mapacalcine is a small protein (Mr=19041) composed of two homologous chains purified from the marine sponge Cliona vastifica. Recently, we demonstrated that it was able to specifically block a Ca2+ channel which could not be related to already described channels on mouse intestinal myocytes. This Ca2+ current was insensitive to the known peptidic and organic calcium channel blockers. Mapacalcine was ineffective on T-type and L-type Ca2+ currents present on rat portal vein myocytes [Morel, Drobecq, Sautière, Tartar, Mironneau, Qar, Lavie, and Hugues (1997) Mol. Pharmacol. 51, 1042-1052]. We report here the preparation and purification of a monoiodo-derivative of mapa-calcine which retains its biological properties. Binding parameters of mapacalcine to its receptors have been characterized on mouse intestinal membranes. It binds to its receptors with a Kd=0. 8 nM, and a maximal binding capacity of 171 fmol/mg of protein on membrane preparations. Our data show that we have prepared a tool that is usable for pharmacological studies of a receptor associated with a new type of calcium channel for which no ligand was available until now."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3310177"xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3310177"xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Hugues M."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Hugues M."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Mironneau J."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Mironneau J."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Morel J.L."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Morel J.L."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Vidalenc P."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/author | "Vidalenc P."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/pages | "177-184"xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/pages | "177-184"xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/title | "125I-Labelled mapacalcine: a specific tool for a pharmacological approach to a receptor associated with a new calcium channel on mouse intestinal membranes."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/title | "125I-Labelled mapacalcine: a specific tool for a pharmacological approach to a receptor associated with a new calcium channel on mouse intestinal membranes."xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/volume | "331"xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://purl.uniprot.org/core/volume | "331"xsd:string |
| http://purl.uniprot.org/citations/9512477 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9512477 |
| http://purl.uniprot.org/citations/9512477 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9512477 |