| http://purl.uniprot.org/citations/9530820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9530820 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9530820 | http://www.w3.org/2000/01/rdf-schema#comment | "Proteins from the calcified exoskeleton of the lobster, Homarus americanus, were extracted and separated by two-dimensional gel-electrophoresis. Electroblotting the proteins onto polyvinylidene difluoride (PVDF) membranes followed by sequence determination gave 16 N-terminal amino-acid sequences and revealed that further eight proteins were N-terminally blocked. The relative molecular mass, M(r), was obtained for most of the electrophoretically separated proteins by means of matrix-assisted laser desorption mass spectrometry (MALDIMS) after electroelution from Coomassie-stained two-dimensional polyacrylamide gels. Eleven proteins were purified from extracts of the exoskeleton by low pressure ion exchange chromatography and reversed-phase high performance chromatography, and their sequences were determined by combined use of Edman degradation and mass spectrometry. Good agreement was obtained between the M(r)-values measured by mass spectrometry and those calculated from the sequences. Five of the sequenced proteins contain two copies of a previously observed 18-residue sequence motif, while a couple of the remaining sequences show similarity to sequences of exoskeletal proteins from shrimps and spiders. Only limited similarity to insect cuticular proteins was observed."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0305-0491(97)00306-4"xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0305-0491(97)00306-4"xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Roepstorff P."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Roepstorff P."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Andersen S.O."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Andersen S.O."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Nousiainen M."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Nousiainen M."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Rafn K."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Rafn K."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Skou L."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/author | "Skou L."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/name | "Comp. Biochem. Physiol."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/name | "Comp. Biochem. Physiol."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/pages | "189-199"xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/pages | "189-199"xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/title | "Characterization of exoskeletal proteins from the American lobster, Homarus americanus."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/title | "Characterization of exoskeletal proteins from the American lobster, Homarus americanus."xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/volume | "119B"xsd:string |
| http://purl.uniprot.org/citations/9530820 | http://purl.uniprot.org/core/volume | "119B"xsd:string |