| http://purl.uniprot.org/citations/9531559 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9531559 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9531559 | http://www.w3.org/2000/01/rdf-schema#comment | "A 130-kD protein that coimmunoprecipitates with the tight junction protein ZO-1 was bulk purified from Madin-Darby canine kidney (MDCK) cells and subjected to partial endopeptidase digestion and amino acid sequencing. A resulting 19-amino acid sequence provided the basis for screening canine cDNA libraries. Five overlapping clones contained a single open reading frame of 2,694 bp coding for a protein of 898 amino acids with a predicted molecular mass of 98,414 daltons. Sequence analysis showed that this protein contains three PSD-95/SAP90, discs-large, ZO-1 (PDZ) domains, a src homology (SH3) domain, and a region similar to guanylate kinase, making it homologous to ZO-1, ZO-2, the discs large tumor suppressor gene product of Drosophila, and other members of the MAGUK family of proteins. Like ZO-1 and ZO-2, the novel protein contains a COOH-terminal acidic domain and a basic region between the first and second PDZ domains. Unlike ZO-1 and ZO-2, this protein displays a proline-rich region between PDZ2 and PDZ3 and apparently contains no alternatively spliced domain. MDCK cells stably transfected with an epitope-tagged construct expressed the exogenous polypeptide at an apparent molecular mass of approximately 130 kD. Moreover, this protein colocalized with ZO-1 at tight junctions by immunofluorescence and immunoelectron microscopy. In vitro affinity analyses demonstrated that recombinant 130-kD protein directly interacts with ZO-1 and the cytoplasmic domain of occludin, but not with ZO-2. We propose that this protein be named ZO-3."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.141.1.199"xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.141.1.199"xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Gu L."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Gu L."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Wittchen E.S."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Wittchen E.S."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Haskins J."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Haskins J."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Stevenson B.R."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Stevenson B.R."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Hibbard J."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/author | "Hibbard J."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/pages | "199-208"xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/pages | "199-208"xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/title | "ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/title | "ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin."xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/volume | "141"xsd:string |
| http://purl.uniprot.org/citations/9531559 | http://purl.uniprot.org/core/volume | "141"xsd:string |