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http://purl.uniprot.org/citations/9547339http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9547339http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9547339http://www.w3.org/2000/01/rdf-schema#comment"Epithelial cells of the alimentary tract play a central role in mucosal immunophysiology. Pathogens and/or agonists that interact with mucosal surfaces often elicit epithelial responses that upregulate inflammation. Therefore, it was of interest to explore potential epithelial targeted antiinflammatory signals. Here we identified and sequenced a human enterocyte lipoxin (LX) A4 [5(S), 6(R),15(S)-trihydroxy-7,9,13-trans-11-cis eicosatetraenoic acid] receptor, and demonstrate that transcription of this receptor was controlled by cytokines, of which lymphocyte-derived interleukin (IL)-13 and interferon gamma were the most potent. When lipoxins and LXA4 stable analogs were evaluated for enterocyte functional as well as immune responses, lipoxins sharply inhibited TNF-alpha-induced IL-8 release but did not alter either barrier function or agonist-stimulated chloride secretion. 15R/S-methyl-LXA4 and 16-phenoxy-LXA4 each attenuated (IC50 approximately 10 nM) IL-8 release. Cyclooxygenase (COX) II is emerging as an important component in wound healing and proliferation in intestinal epithelia and when acetylated by acetylsalicylic acid (aspirin) initiates the biosynthesis of a LXA4 receptor ligand. We therefore determined whether colonic cell lines (HT-29 Cl.19A, Caco-2, or T84) express the COX II isozyme. Results for RT-PCR and Western blot analysis showed that COX I as well as an IL-1beta- and TNF-alpha-inducible COX II are expressed in HT-29 Cl.19A. In addition, aspirin-treated enterocytes generated 15R-HETE, a precursor of 15-epi-LXA4 biosynthesis, whose potent bioactions were mimicked by the stable analog 15R/S-methyl-LXA4. Taken together, these results identify an endogenous pathway for downregulating mucosal inflammatory events and suggest a potential therapeutic benefit for LXA4 stable analogs."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.org/dc/terms/identifier"doi:10.1084/jem.187.8.1285"xsd:string
http://purl.uniprot.org/citations/9547339http://purl.org/dc/terms/identifier"doi:10.1084/jem.187.8.1285"xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Gewirtz A."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Gewirtz A."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Serhan C.N."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Serhan C.N."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Gronert K."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Gronert K."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Madara J.L."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/author"Madara J.L."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/name"J. Exp. Med."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/name"J. Exp. Med."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/pages"1285-1294"xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/pages"1285-1294"xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/title"Identification of a human enterocyte lipoxin A4 receptor that is regulated by interleukin (IL)-13 and interferon gamma and inhibits tumor necrosis factor alpha-induced IL-8 release."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/title"Identification of a human enterocyte lipoxin A4 receptor that is regulated by interleukin (IL)-13 and interferon gamma and inhibits tumor necrosis factor alpha-induced IL-8 release."xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/volume"187"xsd:string
http://purl.uniprot.org/citations/9547339http://purl.uniprot.org/core/volume"187"xsd:string
http://purl.uniprot.org/citations/9547339http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9547339
http://purl.uniprot.org/citations/9547339http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9547339
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