| http://purl.uniprot.org/citations/9564049 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9564049 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9564049 | http://www.w3.org/2000/01/rdf-schema#comment | "In extracts of human cells, base-base mismatches and small insertion/deletion loops are bound primarily by hMutSalpha, a heterodimer of hMSH2 and hMSH6 (also known as GTBP or p160). Recombinant hMutSalpha bound a G/T mismatch-containing oligonucleotide with an apparent dissociation constant Kd = 2.6 nM, while its affinity for a homoduplex substrate was >20-fold lower. In the presence of ATP, hMutSalpha dissociated from mismatched oligonucleotide substrates, and this reaction was attenuated by mutating the conserved lysine in the ATP-binding domains of hMSH6, hMSH2 or both to arginine. Surprisingly, this reaction required only ATP binding, not hydrolysis. The ATPase activity of hMutSalpha variants carrying the Lys-->Arg mutation in hMSH2 or in hMSH6 was severely affected, but these mutants were still proficient in mismatch binding and were able to complement, albeit to different extents, mismatch repair-deficient cell extracts. The mismatch binding-proficient, ATPase-deficient double mutant was inactive in the complementation assay and its presence in repair-proficient extracts was inhibitory. We conclude that although the ATPase activity of hMutSalpha is dispensible for mismatch binding, it is required for mismatch correction."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.9.2677"xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.9.2677"xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Jiricny J."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Jiricny J."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Palombo F."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Palombo F."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Iaccarino I."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Iaccarino I."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Marra G."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/author | "Marra G."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/pages | "2677-2686"xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/pages | "2677-2686"xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/title | "hMSH2 and hMSH6 play distinct roles in mismatch binding and contribute differently to the ATPase activity of hMutSalpha."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/title | "hMSH2 and hMSH6 play distinct roles in mismatch binding and contribute differently to the ATPase activity of hMutSalpha."xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/9564049 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9564049 |
| http://purl.uniprot.org/citations/9564049 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9564049 |