| http://purl.uniprot.org/citations/9570313 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9570313 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9570313 | http://www.w3.org/2000/01/rdf-schema#comment | "Cyclophilins (Cyps) catalyze the cis/trans isomerization of peptidyl-prolyl bonds, a rate-limiting step in protein folding. In some cases, cyclophilins have also been shown to form stable complexes with specific proteins in vivo and may thus also act as chaperone-like molecules. We have characterized the 20kD protein of the spliceosomal 25S [U4/U6.U5] tri-snRNP complex from HeLa cells and show that it is a novel human cyclophilin (denoted SnuCyp-20). Purified [U4/U6.U5] tri-snRNPs, but not U1, U2, or U5 snRNPs, exhibit peptidyl-prolyl cis/trans isomerase activity in vitro, which is cyclosporin A-sensitive, suggesting that SnuCyp-20 is an active isomerase. Consistent with its specific association with tri-snRNPs in vitro, immunofluorescence microscopy studies showed that SnuCyp-20 is predominantly located in the nucleus, where it colocalizes in situ with typical snRNP-containing structures referred to as nuclear speckles. As a first step toward the identification of possible targets of SnuCyp-20, we have investigated the interaction of SnuCyp-20 with other proteins of the tri-snRNP. Fractionation of RNA-free protein complexes dissociated from isolated tri-snRNPs by treatment with high salt revealed that SnuCyp-20 is part of a biochemically stable heteromer containing additionally the U4/U6-specific 60kD and 90kD proteins. By coimmunoprecipitation experiments performed with in vitro-translated proteins, we could further demonstrate a direct interaction between SnuCyp-20 and the 60kD protein, but failed to detect a protein complex containing the 90kD protein. The formation of a stable SnuCyp-20/60kD/90kD heteromer may thus require additional factors not present in our in vitro reconstitution system. We discuss possible roles of SnuCyp-20 in the assembly of [U4/U6.U5] tri-snRNPs and/or in conformational changes occurring during the splicing process."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Lane W.S."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Lane W.S."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Luehrmann R."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Teigelkamp S."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Teigelkamp S."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Achsel T."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Achsel T."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Mundt C."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Mundt C."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Cronshagen U."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Cronshagen U."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Goethel S.-F."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Goethel S.-F."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Marahiel M."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/author | "Marahiel M."xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/pages | "127-141"xsd:string |
| http://purl.uniprot.org/citations/9570313 | http://purl.uniprot.org/core/pages | "127-141"xsd:string |