| http://purl.uniprot.org/citations/9576783 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9576783 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9576783 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9576783 | http://www.w3.org/2000/01/rdf-schema#comment | "The four major oligomeric reaction products from saponified modified hairy regions (MHR-S) from apple, produced by recombinant rhamnogalacturonan (RG) alpha-L-rhamnopyranosyl-(1, 4)-alpha-D-galactopyranosyluronide lyase (rRG-lyase) from Aspergillus aculeatus, were isolated and characterized by 1H-nuclear magnetic resonance spectroscopy. They contain an alternating RG backbone with a degree of polymerization of 4, 6, 8, and 10 and with an alpha-Delta-(4,5)-unsaturated D-galactopyranosyluronic acid at the nonreducing end and an L-rhamnopyranose at the reducing end. L-Rhamnopyranose units are substituted at C-4 with beta-galactose. The maximum reaction rate of rRG-lyase toward MHR-S at pH 6.0 and 31 degreesC was 28 units mg-1. rRG-lyase and RG-hydrolase cleave the same alternating RG I subunit in MHR. Both of these enzymes fragment MHR by a multiple attack mechanism. The catalytic efficiency of rRG-lyase for MHR increases with decreasing degree of acetylation. Removal of arabinose side chains improves the action of rRG-lyase toward MHR-S. In contrast, removal of galactose side chains decreased the catalytic efficiency of rRG-lyase. Native RG-lyase was purified from A. aculeatus, characterized, and found to be similar to the rRG-lyase expressed in Aspergillus oryzae."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.117.1.141"xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.117.1.141"xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Voragen A.G."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Voragen A.G."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Schols H.A."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Schols H.A."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Beldman G."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Beldman G."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Colquhoun I.J."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Colquhoun I.J."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Mutter M."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Mutter M."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Bakx E.J."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/author | "Bakx E.J."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/pages | "141-152"xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/pages | "141-152"xsd:string |
| http://purl.uniprot.org/citations/9576783 | http://purl.uniprot.org/core/title | "Characterization of recombinant rhamnogalacturonan alpha-L-rhamnopyranosyl-(1,4)-alpha-D-galactopyranosyluronide lyase from Aspergillus aculeatus. An enzyme that fragments rhamnogalacturonan I regions of pectin."xsd:string |