| http://purl.uniprot.org/citations/9610360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9610360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9610360 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9610360 | http://www.w3.org/2000/01/rdf-schema#comment | "Three metallo forms of peptide deformylase (PDF, EC 3.5.1.31) of Escherichia coli were prepared and crystallized (space group C2, diffraction limit 1.9 A) for initiating the X-ray structure determination of the metal center in correlation with the catalytic functionality of this enzyme. The native Fe2+ containing enzyme species was directly isolated from overproducing bacteria by using catalase as a buffer additive, which stabilizes the catalytic activity against oxidative destruction. The Ni2+ containing form, which is oxygen-insensitive, was obtained by metal exchange with free Ni2+ and found to be catalytically equally effective (kcat/KM = 10(5) M-1 s-1 for N-formyl-Met-Ala). The Zn2+ form, prepared from the apoenzyme or by displacement of bound Ni2+ by free Zn2+, proved virtually inactive."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1998.8616"xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1998.8616"xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Becker A."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Becker A."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Schlichting I."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Schlichting I."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Kabsch W."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Kabsch W."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Schultz S."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Schultz S."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Groche D."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Groche D."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Wagner A.F."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/author | "Wagner A.F."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/pages | "342-346"xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/pages | "342-346"xsd:string |
| http://purl.uniprot.org/citations/9610360 | http://purl.uniprot.org/core/title | "Isolation and crystallization of functionally competent Escherichia coli peptide deformylase forms containing either iron or nickel in the active site."xsd:string |