| http://purl.uniprot.org/citations/9613572 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9613572 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9613572 | http://www.w3.org/2000/01/rdf-schema#comment | "Open reading frames in the genome of Saccharomyces cerevisiae were screened for potential glycosylphosphatidylinositol (GPI)-attached proteins. The identification of putative GPI-attached proteins was based on three criteria: the presence of a GPI-attachment signal sequence, a signal sequence for secretion and a serine- or threonine-rich sequence. In all, 53 ORFs met these three criteria and 38 were further analyzed as follows. The sequence encoding the 40 C-terminal amino acids of each was fused with the structural gene for a reporter protein consisting of a secretion signal, alpha-galactosidase and a hemagglutinin (HA) epitope, and examined for the ability to become incorporated into the cell wall. On this basis, 14 of fusion proteins were classified as GPI-dependent cell wall proteins because cells expressing these fusion proteins: (i) had high levels of alpha-galactosidase activity on their surface; (ii) released significant amounts of the fusion proteins from the membrane on treatment with phosphatidylinositol-specific phospholipase C (PI-PLC); and (iii) released fusion proteins from the cell wall following treatment with laminarinase. Of the 14 identified putative GPI-dependent cell wall proteins, 12 had novel ORFs adjacent to their GPI-attachment signal sequence. Amino acid sequence alignment of the C-terminal sequences of the 12 ORFs, together with those of known cell wall proteins, reveals some sequence similarities among them."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.org/dc/terms/identifier | "doi:10.1007/s004380050706"xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.org/dc/terms/identifier | "doi:10.1007/s004380050706"xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Baba M."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Baba M."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Hamada K."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Hamada K."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Arisawa M."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Arisawa M."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Kitada K."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Kitada K."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Fukuchi S."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/author | "Fukuchi S."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/name | "Mol. Gen. Genet."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/name | "Mol. Gen. Genet."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/pages | "53-59"xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/pages | "53-59"xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/title | "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/title | "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/volume | "258"xsd:string |
| http://purl.uniprot.org/citations/9613572 | http://purl.uniprot.org/core/volume | "258"xsd:string |