| http://purl.uniprot.org/citations/9636051 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9636051 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9636051 | http://www.w3.org/2000/01/rdf-schema#comment | "Calciseptine and FS2 are 60-amino acid polypeptides, isolated from venom of the black mamba (Dendroaspis polylepis polylepis), that block voltage-dependent L-type Ca2+ channels. We predicted that these polypeptides contain an identical functional site between residues 43 and 46 by searching for proline residues that mark the flanks of protein-protein interaction sites [Kini, R. M., and Evans, H. J. (1966) FEBS Lett. 385, 81-86]. The predicted Ca2+ channel binding site also occurs in closely related toxins, C10S2C2 and S4C8. Therefore, it is likely that these toxins also will block L-type Ca2+ channels. To test the proposed binding site on calciseptine and FS2, an eight-residue peptide, named L-calchin (L-type calcium channel inhibitor), was synthesized and examined for biological activity. As expected for an L-type Ca2+ channel blocker, L-calchin reduced peak systolic and developed pressure in isolated rat heart Langendorff preparations without affecting diastolic pressure or heart rate. Furthermore, L-calchin caused a voltage-independent block of L-type Ca2+ channel currents in whole-cell patch-clamped rabbit ventricular myocytes. Thus the synthetic peptide exhibits the L-type Ca2+ channel blocking properties of the parent molecules, calciseptine and FS2, but with a lower potency. These results strongly support the identification of a site in calciseptine and FS2 that is important for binding to L-type Ca2+ channels and reinforce the importance of proline brackets flanking protein-protein interaction sites."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi9802723"xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi9802723"xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Baumgarten C.M."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Baumgarten C.M."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Kini R.M."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Kini R.M."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Caldwell R.A."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Caldwell R.A."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Evans H.J."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Evans H.J."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Feher J.J."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Feher J.J."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Wu Q.Y."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/author | "Wu Q.Y."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/pages | "9058-9063"xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/pages | "9058-9063"xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/title | "Flanking proline residues identify the L-type Ca2+ channel binding site of calciseptine and FS2."xsd:string |
| http://purl.uniprot.org/citations/9636051 | http://purl.uniprot.org/core/title | "Flanking proline residues identify the L-type Ca2+ channel binding site of calciseptine and FS2."xsd:string |