| http://purl.uniprot.org/citations/9636715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9636715 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9636715 | http://www.w3.org/2000/01/rdf-schema#comment | "Raphanus sativus Antifungal Protein 1 (Rs-AFP1) is a 51 amino acid residue plant defensin isolated from radish (Raphanus sativus L.) seeds. The three-dimensional structure in aqueous solution has been determined from two-dimensional 1H NMR data recorded at 500 MHz using the DIANA/REDAC calculation protocols. Experimental constraints consisted of 787 interproton distances extracted from NOE cross-peaks, 89 torsional constraints from 106 vicinal interproton coupling constants and 32 stereospecific assignments of prochiral protons. Further refinement by simulated annealing resulted in a set of 20 structures having pairwise root-mean-square differences of 1.35(+/-0.35) A over the backbone heavy atoms and 2.11(+/-0.46) A over all heavy atoms. The molecule adopts a compact globular fold comprising an alpha-helix from Asn18 till Leu28 and a triple-stranded beta-sheet (beta 1 = Lys2-Arg6, beta 2 = His33-Tyr38 and beta 3 = His43-Pro50). The central strand of this beta-sheet is connected by two disulfide bridges (Cys21-Cys45 and Cys25-Cys47) to the alpha-helix. The connection between beta-strand 2 and 3 is formed by a type VIa beta-turn. Even the loop (Pro7 to Asn17) between beta-strand 1 and the alpha-helix is relatively well defined. The structure of Raphanus sativus Antifungal Protein 1 features all the characteristics of the "cysteine stabilized alpha beta motif". A comparison of the complete structure and of the regions important for interaction with the fungal receptor according to a mutational study, is made with the structure of gamma-thionin, a plant defensin that has no antifungal activity. It is concluded that this interaction is both electrostatic and specific, and some possible scenarios for the mode of action are given."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.org/dc/terms/identifier | "doi:10.1006/jmbi.1998.1767"xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.org/dc/terms/identifier | "doi:10.1006/jmbi.1998.1767"xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Broekaert W.F."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Broekaert W.F."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Borremans F.A.M."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Borremans F.A.M."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Fant F."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Fant F."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Vranken W.F."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/author | "Vranken W.F."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/name | "J. Mol. Biol."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/pages | "257-270"xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/pages | "257-270"xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/title | "Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/title | "Determination of the three-dimensional solution structure of Raphanus sativus antifungal protein 1 by 1H NMR."xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://purl.uniprot.org/core/volume | "279"xsd:string |
| http://purl.uniprot.org/citations/9636715 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9636715 |
| http://purl.uniprot.org/citations/9636715 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9636715 |