| http://purl.uniprot.org/citations/9694873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9694873 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9694873 | http://www.w3.org/2000/01/rdf-schema#comment | "We previously reported the cDNA cloning and characterization of a mammalian mitochondrial GrpE protein ( approximately 21 kDa, mt-GrpE#1) and now provide evidence for the presence of distinct cytosolic ( approximately 40 kDa), microsomal ( approximately 50 kDa), and additional mitochondrial ( approximately 22 kDa, mt-GrpE#2) GrpE-like members. While a cytosolic GrpE-like protein has recently been identified, the demonstration of both a microsomal and a second mitochondrial GrpE-like member represents the first in any biological system. Investigation of the microsomal and two mitochondrial GrpE-like proteins revealed that they bound specifically to Escherichia coli DnaK, and the complexes formed were not disrupted in the presence of 0.5 M salt but were readily dissociated in the presence of 5 mM ATP. The functional integrity of mt-GrpE#1 and #2 was verified by their ability to specifically interact with and stimulate the ATPase activity of mammalian mitochondrial Hsp70 (mt-Hsp70). Analysis of the cDNA sequences encoding the two mammalian mitochondrial GrpE-like proteins revealed approximately 47% positional identity at the amino acid level, the presence of a highly conserved mitochondrial leader sequence, and putative destabilization elements within the 3'-untranslated region of the mt-GrpE#2 transcript which are not present in the mt-GrpE#1 transcript. A constitutive expression of both mitochondrial GrpE-like transcripts in 22 distinct mouse tissues was observed but possible different post-transcriptional regulation of the mt-GrpE#1 and #2 transcripts may confer a different expression pattern of the encoded proteins."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.33.21169"xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.33.21169"xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Hoej P.B."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Hoej P.B."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Hoogenraad N.J."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Hoogenraad N.J."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Naylor D.J."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Naylor D.J."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Stines A.P."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/author | "Stines A.P."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/pages | "21169-21177"xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/pages | "21169-21177"xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/title | "Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the co-chaperones of specific Hsp70 members."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/title | "Evidence for the existence of distinct mammalian cytosolic, microsomal, and two mitochondrial GrpE-like proteins, the co-chaperones of specific Hsp70 members."xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9694873 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9694873 |
| http://purl.uniprot.org/citations/9694873 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9694873 |