| http://purl.uniprot.org/citations/9722581 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9722581 | http://www.w3.org/2000/01/rdf-schema#comment | "Affinity purification of a phage-displayed library, expressing random peptide 12-mers at the N terminus of protein III, has identified 10 distinct novel sequences which bind troponin C specifically. The troponin C-selected peptides yield a consensus binding sequence of (V/L)(D/E)XLKXXLXXLA. Sequence comparison revealed as much as a 62.5% similarity between phiT5, the peptide sequence of the phage clone with the highest level of binding to troponin C, and the N-terminal region of troponin I isoforms. Biotinylated peptides corresponding to library-derived sequences and similar sequences from various isoforms of troponin I were synthesized shown to bind troponin C specifically. Alkaline phosphatase fusion proteins of two of the phage clone sequences bound troponin C specifically, and were specifically competed by both library-derived and native troponin I peptides. Measurement of equilibrium dissociation constants of the peptides by surface plasmon resonance yielded dissociation constants for troponin C as low as 0.43 microM for pT5; in contrast, dissociation constants for calmodulin were greater than 6 microM for all peptides studied. Nondenaturing polyacrylamide gel electrophoresis demonstrated that pT5 formed a stable complex with troponin C in the presence of calcium. We also found that the pT5 peptide inhibited the maximal calcium-activated tension of rabbit psoas muscle fibers."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.36.23448"xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/author | "Kay B.K."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/author | "Lombardo C.R."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/author | "Schachat F."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/author | "Brandt P.W."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/author | "Pierce H.H."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/pages | "23448-23453"xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/title | "Identification of troponin C antagonists from a phage-displayed random peptide library."xsd:string |
| http://purl.uniprot.org/citations/9722581 | http://purl.uniprot.org/core/volume | "273"xsd:string |
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