| http://purl.uniprot.org/citations/9724629 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9724629 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9724629 | http://www.w3.org/2000/01/rdf-schema#comment | "Biosynthesis of glycosylphosphatidylinositol and N-glycan precursor is dependent upon a mannosyl donor, dolichol phosphate-mannose (DPM). The Thy-1negative class E mutant of mouse lymphoma and Lec15 mutant Chinese hamster ovary (CHO) cells are incapable of DPM synthesis. The class E mutant is defective in the DPM1 gene which encodes a mammalian homologue of Saccharomyces cerevisiae Dpm1p that is a DPM synthase, whereas Lec15 is a different mutant, indicating that mammalian DPM1 is not sufficient for DPM synthesis. Here we report expression cloning of a new gene, DPM2, which is defective in Lec15 cells. DPM2, an 84 amino acid membrane protein expressed in the endoplasmic reticulum (ER), makes a complex with DPM1 that is essential for the ER localization and stable expression of DPM1. Moreover, DPM2 enhances binding of dolichol phosphate, a substrate of DPM synthase. Mammalian DPM1 is catalytic because a fusion protein of DPM1 that was stably expressed in the ER synthesized DPM without DPM2. Therefore, biosynthesis of DPM in mammalian cells is regulated by DPM2."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.17.4920"xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.17.4920"xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Maeda Y."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Maeda Y."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Kinoshita T."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Kinoshita T."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Ohishi K."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Ohishi K."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Watanabe R."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Watanabe R."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Tomita S."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/author | "Tomita S."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/pages | "4920-4929"xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/pages | "4920-4929"xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/title | "DPM2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of DPM1, and binding of dolichol phosphate."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/title | "DPM2 regulates biosynthesis of dolichol phosphate-mannose in mammalian cells: correct subcellular localization and stabilization of DPM1, and binding of dolichol phosphate."xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/volume | "17"xsd:string |
| http://purl.uniprot.org/citations/9724629 | http://purl.uniprot.org/core/volume | "17"xsd:string |