| http://purl.uniprot.org/citations/9724729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9724729 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9724729 | http://www.w3.org/2000/01/rdf-schema#comment | "Lysyl hydroxylase (EC 1.14.11.4), a homodimer, catalyzes the formation of hydroxylysine in collagens. Recently, an isoenzyme termed lysyl hydroxylase 2 has been cloned from human sources [M. Valtavaara, H. Papponen, A.-M. Pirttilä, K. Hiltunen, H. Helander and R. Myllylä (1997) J. Biol. Chem. 272, 6831-6834]. We report here on the cloning of a third human lysyl hydroxylase isoenzyme, termed lysyl hydroxylase 3. The cDNA clones encode a 738 amino acid polypeptide, including a signal peptide of 24 residues. The overall amino acid sequence identity between the processed human lysyl hydroxylase 3 and 1 polypeptides is 59%, and that between the processed lysyl hydroxylase 3 and 2 polypeptides is 57%, whereas the identity to the processed Caenorhabditis elegans polypeptide is only 45%. All four recently identified critical residues at the catalytic site, two histidines, one aspartate, and one arginine, are conserved in all these polypeptides. The mRNA for lysyl hydroxylase 3 was found to be expressed in a variety of tissues, but distinct differences appear to exist in the expression patterns of the three isoenzyme mRNAs. Recombinant lysyl hydroxylase 3 expressed in insect cells by means of a baculovirus vector was found to be more soluble than lysyl hydroxylase 1 expressed in the same cell type. No differences in catalytic properties were found between the recombinant lysyl hydroxylase 3 and 1 isoenzymes. Deficiency in lysyl hydroxylase 1 activity is known to cause the type VI variant of the Ehlers-Danlos syndrome, and it is therefore possible that deficiency in lysyl hydroxylase 3 activity may lead to some other variant of this syndrome or to some other heritable connective tissue disorder."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.95.18.10482"xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.95.18.10482"xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Ala-Kokko L."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Ala-Kokko L."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Kivirikko K.I."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Kivirikko K.I."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Rautavuoma K."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Rautavuoma K."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Kosonen T."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Kosonen T."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Passoja K."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/author | "Passoja K."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/pages | "10482-10486"xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/pages | "10482-10486"xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/title | "Cloning and characterization of a third human lysyl hydroxylase isoform."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/title | "Cloning and characterization of a third human lysyl hydroxylase isoform."xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/volume | "95"xsd:string |
| http://purl.uniprot.org/citations/9724729 | http://purl.uniprot.org/core/volume | "95"xsd:string |