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http://purl.uniprot.org/citations/9727399http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9727399http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9727399http://www.w3.org/2000/01/rdf-schema#comment"

Purpose

To characterize the cDNA and the organization of the gene encoding the cone-specific gamma subunit of transducin (Tgamma c) and to examine this gene as a candidate for the recessively inherited cone photoreceptor degeneration in the cd dog.

Methods

Canine Tgamma c cDNA was cloned and sequenced. Polymerase chain reaction (PCR) was used to define the Tgamma c gene structure, northern blot analysis to examine the level of expression of Tgamma c mRNA in control and cd-affected retinas, and immunocytochemistry to determine the presence and localization of Tgamma c in normal and cd retinas.

Results

Immunocytochemical results showed Tgamma c localized to cone photoreceptor outer segments in the normal retina, whereas no Tgamma c immunoreactivity was observed in the cd retinas. However, the level of transcription and the primary structure of the cloned cDNA coding for the 69-amino acid protein were identical in retinas from wild-type and affected dogs.

Conclusions

Although Tgamma c immunoreactivity was specifically absent in the cd dog retina, no differences were detected between normal and cd retinas in the nucleotide sequence of Tgamma c mRNA or in its synthesis. These results indicate that a mutation in the Tgamma c gene may not be causally associated with the cd dog disease. These findings suggest that possible abnormalities in posttranslational modification of Tgamma c or defective assembly of the transducin alphabetagamma complex could lead to rapid degradation of Tgamma c."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Acland G.M."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Acland G.M."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Aguirre G.D."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Aguirre G.D."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Farber D.B."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Farber D.B."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Akhmedov N.B."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Akhmedov N.B."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Pearce-Kelling S."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Pearce-Kelling S."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Piriev N.I."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/author"Piriev N.I."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/name"Invest. Ophthalmol. Vis. Sci."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/name"Invest. Ophthalmol. Vis. Sci."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/pages"1775-1781"xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/pages"1775-1781"xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/title"Canine cone transducin-gamma gene and cone degeneration in the cd dog."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/title"Canine cone transducin-gamma gene and cone degeneration in the cd dog."xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/volume"39"xsd:string
http://purl.uniprot.org/citations/9727399http://purl.uniprot.org/core/volume"39"xsd:string
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