Results

RDF/XMLNTriplesTurtleShow queryShare ▶
SubjectPredicateObject
http://purl.uniprot.org/citations/9730838http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9730838http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9730838http://www.w3.org/2000/01/rdf-schema#comment"Cobalamin-dependent methionine synthase catalyzes the transfer of a methyl group from methyltetrahydrofolate to homocysteine, generating tetrahydrofolate and methionine. During this primary turnover cycle, the enzyme alternates between the active methylcobalamin and cob(I)alamin forms of the enzyme. Formation of the cob(II)alamin prosthetic group by oxidation of cob(I)alamin or photolysis of methylcobalamin renders the enzyme inactive. Methionine synthase from E. coli catalyzes its own reactivation by a reductive methylation that involves electron transfer from reduced flavodoxin and methyl transfer from AdoMet. This process has been proposed to involve formation of a transient cob(I)alamin intermediate that is then trapped by methyl transfer from AdoMet. During aerobic growth of E. coli, electrons for this process are ultimately derived from NADPH, and electron transfer does not generate a detectable level of cob(I)alamin due to the large potential difference between the NADPH/NADP+ couple and the cob(I)alamin/cob(II)alamin couple. In this paper, we show that even in the presence of the strong reductant flavodoxin hydroquinone, cob(I)alamin is not observed as a significant intermediate. We demonstrate, however, that this is due to a rate-limiting reorganization of the cobalt ligand environment from five-coordinate to four-coordinate cob(II)alamin. Mutation of aspartate 757 to glutamate results in a cob(II)alamin enzyme that is approximately 70% four-coordinate, and reductive methylation of this enzyme using flavodoxin hydroquinone as the electron donor proceeds through a kinetically competent cob(I)alamin intermediate. Furthermore, wild-type cob(I)alamin enzyme produced by chemical reduction reacts with AdoMet in a kinetically competent reaction. We provide evidence that methyl transfer from AdoMet to cob(I)alamin enzyme results initially in formation of a five-coordinate methylcobalamin enzyme that slowly decays to the active six-coordinate methylcobalamin enzyme. We propose a kinetic scheme for reductive methylation of wild-type cob(II)alamin enzyme by adenosylmethionine and flavodoxin hydroquinone in which slow conformational changes mask the relatively fast electron and methyl transfer steps."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.org/dc/terms/identifier"doi:10.1021/bi9808565"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.org/dc/terms/identifier"doi:10.1021/bi9808565"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Ludwig M.L."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Ludwig M.L."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Matthews R.G."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Matthews R.G."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Jarrett J.T."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Jarrett J.T."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Hoover D.M."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/author"Hoover D.M."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/name"Biochemistry"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/name"Biochemistry"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/pages"12649-12658"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/pages"12649-12658"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/title"The mechanism of adenosylmethionine-dependent activation of methionine synthase: a rapid kinetic analysis of intermediates in reductive methylation of Cob(II)alamin enzyme."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/title"The mechanism of adenosylmethionine-dependent activation of methionine synthase: a rapid kinetic analysis of intermediates in reductive methylation of Cob(II)alamin enzyme."xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/volume"37"xsd:string
http://purl.uniprot.org/citations/9730838http://purl.uniprot.org/core/volume"37"xsd:string
http://purl.uniprot.org/citations/9730838http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9730838
http://purl.uniprot.org/citations/9730838http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9730838
▶