| http://purl.uniprot.org/citations/9731776 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9731776 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9731776 | http://www.w3.org/2000/01/rdf-schema#comment | "The catalytic mechanism of 'retaining' beta-glycosidases has been the subject of considerable interest and debate for many years. The visualization of a covalent glycosyl enzyme intermediate by X-ray crystallography was first accomplished with a saccharide substrate substituted with fluorine at its 2-position. The structure implicated major roles for residue His 205 and for the 2-hydroxyl position of the proximal saccharide in binding and catalysis. Here we have studied the kinetic behavior of various His 205 mutants. One of these mutants, a double mutant H205N/E127A, has been used to stabilize a covalent glycosyl-enzyme intermediate involving an unsubstituted sugar, permitting crystallographic analysis of the interactions between its 2-hydroxyl group and the enzyme."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.org/dc/terms/identifier | "doi:10.1038/1852"xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.org/dc/terms/identifier | "doi:10.1038/1852"xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Withers S.G."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Withers S.G."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Warren R.A."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Warren R.A."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Rose D.R."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Rose D.R."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Birsan C."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Birsan C."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Nitz M."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Nitz M."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Notenboom V."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/author | "Notenboom V."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/name | "Nat. Struct. Biol."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/name | "Nat. Struct. Biol."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/pages | "812-818"xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/pages | "812-818"xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/title | "Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants."xsd:string |
| http://purl.uniprot.org/citations/9731776 | http://purl.uniprot.org/core/title | "Insights into transition state stabilization of the beta-1,4-glycosidase Cex by covalent intermediate accumulation in active site mutants."xsd:string |