| http://purl.uniprot.org/citations/9733793 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9733793 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9733793 | http://www.w3.org/2000/01/rdf-schema#comment | "The basis for the unusual properties of the catalytic subunit (C) of ram sperm cAMP-dependent protein kinase was investigated. Ram sperm C was purified and found by mass spectrometry (MS) to be approximately 890 Da smaller than Calpha, the predominant somatic isoform. Partial internal amino acid sequence from ram sperm C was an exact match to that of bovine Calpha, but differed from the predicted sequences for the Cbeta and Cgamma isoforms. MS analysis of 2-nitro-5-thiocyanatobenzoic acid fragments showed that the mass difference originated in the amino-terminal region. A unique blocked amino-terminal fragment was isolated from sperm C and sequenced by a combination of tandem mass spectrometry and Edman degradation of a subfragment. The results revealed that the amino-terminal myristate and the first 14 amino acids of Calpha are replaced by an amino-terminal acetate and six different amino acids in sperm C. The predicted mass difference due to these changes is 899 Da. The region of homology between sperm C and Calpha begins at the exon 1/exon 2 boundary in Calpha, suggesting that sperm C results from use of an alternate exon 1 in the Calpha gene. The different amino terminus of sperm C may be related to a unique requirement for localization of the "free" C subunit within the sperm flagellum."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.38.24874"xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.273.38.24874"xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "Witman G.B."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "Witman G.B."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "San Agustin J.T."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "San Agustin J.T."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "Leszyk J.D."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "Leszyk J.D."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "Nuwaysir L.M."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/author | "Nuwaysir L.M."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/pages | "24874-24883"xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/pages | "24874-24883"xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/title | "The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/title | "The catalytic subunit of the cAMP-dependent protein kinase of ovine sperm flagella has a unique amino-terminal sequence."xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://purl.uniprot.org/core/volume | "273"xsd:string |
| http://purl.uniprot.org/citations/9733793 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9733793 |
| http://purl.uniprot.org/citations/9733793 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9733793 |