| http://purl.uniprot.org/citations/9742218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9742218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9742218 | http://www.w3.org/2000/01/rdf-schema#comment | "We identified SH2-Balpha as an insulin-receptor-binding protein based on interaction screening in yeast hybrid systems and co-precipitation in cells. SH2-Balpha contains pleckstrin-homology ('PH') and Src homology 2 (SH2) domains and is closely related to APS (adapter protein with a PH domain and an SH2 domain) and lnk, adapter proteins first identified in lymphocytes. SH2-Balpha is ubiquitously expressed and is present in rat epididymal adipose tissue, liver and skeletal muscle, physiological sites of insulin action. On SDS/PAGE, SH2-Balpha migrates at a molecular mass of 98 kDa, although the predicted size of SH2-Balpha is 79.6 kDa. Insulin causes an electrophoretic mobility shift. SH2-Balpha can be immunoprecipitated using anti-(insulin receptor) antibody from insulin-stimulated cells. Anti-phosphotyrosine antibody or the growth factor receptor-binding protein 2 (Grb2) SH2 domain precipitate SH2-Balpha after insulin stimulation, suggesting that SH2-Balpha is tyrosine-phosphorylated and may be a substrate for the insulin receptor. The SH2-Balpha SH2 domain did not interact with insulin-receptor substrate (IRS) proteins or epidermal-growth-factor receptor. Mutation of the juxtamembrane and C-terminus of the insulin receptor did not abolish the interaction with the SH2 domain. This was further confirmed using a panel of activation-loop single point mutants where mutation of Tyr1158, Tyr1162 and Tyr1163 abolished interaction. Thus SH2-Balpha is a likely component in the insulin-signalling pathway and may function as an alternative signalling protein by interacting with the activation loop of the insulin-receptor cytoplasmic domain."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3350103"xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.org/dc/terms/identifier | "doi:10.1042/bj3350103"xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/author | "Kotani K."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/author | "Kotani K."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/author | "Pillay T.S."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/author | "Pillay T.S."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/author | "Wilden P."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/author | "Wilden P."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/name | "Biochem. J."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/pages | "103-109"xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/pages | "103-109"xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/title | "SH2-Balpha is an insulin-receptor adapter protein and substrate that interacts with the activation loop of the insulin-receptor kinase."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/title | "SH2-Balpha is an insulin-receptor adapter protein and substrate that interacts with the activation loop of the insulin-receptor kinase."xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/volume | "335"xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://purl.uniprot.org/core/volume | "335"xsd:string |
| http://purl.uniprot.org/citations/9742218 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9742218 |
| http://purl.uniprot.org/citations/9742218 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9742218 |
| http://purl.uniprot.org/citations/9742218 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9742218 |
| http://purl.uniprot.org/citations/9742218 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9742218 |