| http://purl.uniprot.org/citations/9748507 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9748507 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9748507 | http://www.w3.org/2000/01/rdf-schema#comment | "Using part of the dnaK gene from Bacillus subtilis as a probe, a 4. 4-kbp SacI-BglII fragment of chromosomal DNA of Bacillus brevis, a protein-hypersecreting bacterium, was cloned. Nucleotide sequencing revealed 3 open reading frames in the order of grpE-dnaK-dnaJ homologues. We purified DnaK protein to homogeneity from B. brevis HPD31 harboring a multi-copy dnaK expression plasmid. Purified DnaK showed ATPase activity which was synergistically stimulated 14-fold by the addition of glutathione S-transferase-DnaJ and glutathione S-transferase-GrpE fusion proteins. DnaK hydrolyzed not only ATP but also CTP, UTP, and GTP at about 40% of the efficiency of ATP. The specific activity of DnaK-ATPase was 7.25x10-3 unit/mg protein (the turnover number against ATP was 0.47 min-1) under our assay conditions. The DnaK dimers dissociated into monomers on addition of ATP, GTP, CTP, UTP and ATPgammaS, but not ADP or AMP. DnaK formed a stable complex with permanently unfolded carboxymethylated alpha-lactalbumin but not with native alpha-lactalbumin, and this complex was dissociated by addition of ATP/Mg. Formation of this complex was inhibited in the presence of inorganic phosphate."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4838(98)00108-3"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0167-4838(98)00108-3"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Mizukami M."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Mizukami M."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Takagi H."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Takagi H."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Yamakawa M."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Yamakawa M."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Tokunaga H."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Tokunaga H."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Tokunaga M."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/author | "Tokunaga M."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/name | "Biochim. Biophys. Acta"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/pages | "65-79"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/pages | "65-79"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/title | "Molecular cloning of the dnaK locus, and purification and characterization of a DnaK protein from Bacillus brevis HPD31."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/title | "Molecular cloning of the dnaK locus, and purification and characterization of a DnaK protein from Bacillus brevis HPD31."xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/volume | "1387"xsd:string |
| http://purl.uniprot.org/citations/9748507 | http://purl.uniprot.org/core/volume | "1387"xsd:string |