| http://purl.uniprot.org/citations/9753431 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9753431 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9753431 | http://www.w3.org/2000/01/rdf-schema#comment | "Ral-specific guanine nucleotide exchange factors RalGDS, Rgl, and Rlf have been suggested to function as intermediates between Ras and Ral pathways by being able to bind Ras proteins through their C-terminal Ras-binding domains (RBD). The RBDs of RalGDS and of the Ser/Thr kinase c-Raf-1 have been shown to have the same tertiary structure. In contrast to the RBDs of Raf and RalGDS, which bind either Ras or Rap with high affinity, Rlf-RBD has a similar affinity for both GTP-binding proteins. To be able to compare these RBDs on a structural level, we have solved the three-dimensional structure of Rlf-RBD by NMR spectroscopy. The overall tertiary structure of Rlf-RBD shows the betabetaalphabetabetaalphabeta-fold of the ubiquitin superfamily and is very similar to that of RalGDS-RBD. The binding interface of Rlf-RBD to Ras was mapped using chemical shift analysis and indicated a binding mode similar to that in the case of Rap.Raf-RBD. However, comparison of the putatively interacting regions revealed structural differences which are proposed to be responsible for the different substrate affinities of Rlf-, RalGDS-, and Raf-RBD."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi9811664"xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.org/dc/terms/identifier | "doi:10.1021/bi9811664"xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Wittinghofer A."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Wittinghofer A."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Bayer P."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Bayer P."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Bauer B."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Bauer B."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Cool R.H."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Cool R.H."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Esser D."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Esser D."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Wolthuis R.M."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/author | "Wolthuis R.M."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/name | "Biochemistry"xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/pages | "13453-13462"xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/pages | "13453-13462"xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/title | "Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf."xsd:string |
| http://purl.uniprot.org/citations/9753431 | http://purl.uniprot.org/core/title | "Structure determination of the Ras-binding domain of the Ral-specific guanine nucleotide exchange factor Rlf."xsd:string |