| http://purl.uniprot.org/citations/9753662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9753662 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9753662 | http://www.w3.org/2000/01/rdf-schema#comment | "gamma-Butyrobetaine hydroxylase (EC 1.14.11.1) is the last enzyme in the biosynthetic pathway of L-carnitine and catalyzes the formation of L-carnitine from gamma-butyrobetaine, a reaction dependent on alpha-ketoglutarate, Fe2+, and oxygen. We report the purification of the protein from rat liver to apparent homogeneity, which allowed N-terminal sequencing using Edman degradation. The obtained amino acid sequence was used to screen the expressed sequence tag database and led to the identification of a human cDNA containing an open reading frame of 1161 base pairs encoding a polypeptide of 387 amino acids with a predicted molecular weight of 44.7 kDa. Heterologous expression of the open reading frame in the yeast Saccharomyces cerevisiae confirmed that the cDNA encodes the human gamma-butyrobetaine hydroxylase. Northern blot analysis showed gamma-butyrobetaine hydroxylase expression in kidney (high), liver (moderate), and brain (very low), while no expression could be detected in the other investigated tissues."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1998.9343"xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.org/dc/terms/identifier | "doi:10.1006/bbrc.1998.9343"xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Ijlst L."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Ijlst L."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Ofman R."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Ofman R."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Wanders R.J.A."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Wanders R.J.A."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Vaz F.M."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "Vaz F.M."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "van Gool S."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/author | "van Gool S."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/name | "Biochem. Biophys. Res. Commun."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/pages | "506-510"xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/pages | "506-510"xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/title | "Carnitine biosynthesis: identification of the cDNA encoding human gamma-butyrobetaine hydroxylase."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/title | "Carnitine biosynthesis: identification of the cDNA encoding human gamma-butyrobetaine hydroxylase."xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/volume | "250"xsd:string |
| http://purl.uniprot.org/citations/9753662 | http://purl.uniprot.org/core/volume | "250"xsd:string |