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http://purl.uniprot.org/citations/9764360http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9764360http://www.w3.org/2000/01/rdf-schema#comment"

Problem

To determine the ontogeny of major histocompatibility complex (MHC) expression and TAP products in mouse embryos.

Method of study

mRNAs encoding MHC and associated molecules were identified by reverse transcriptase-polymerase chain reaction, and the protein products were localized by confocal microscopy.

Results

mRNAs encoding class Ia (H-2Db) and class Ib (Q7/9) were present in one-cell embryos, whereas beta 2-microglobulin (beta 2-m) transcripts were not detected until the two-cell stage. Transporter TAP1, but not TAP2, transcripts were detected only in blastocysts. H-2 class Ia (classical) protein was detected on the surface of two-cell embryos, H-2 class Ib (nonclassical) protein was detected on one-cell embryos, and beta 2-m transcripts were detected on eight-cell embryos; TAP1 protein was present at low levels in the cytoplasm from the one-cell stage onward, increasing in expression in blastocysts.

Conclusions

In mice, MHC class I mRNAs encoding the heavy chain of H-2- and Q7/9-encoding Qa2 molecules are synthesized soon after conception prior to implantation. Similarly, the nonpolymorphic MHC class I-associated molecule beta 2-m also is expressed before implantation. TAP1, but not TAP2, is first detected at the blastocyst stage, thus preceding the onset of TAP2 in embryonic development."xsd:string
http://purl.uniprot.org/citations/9764360http://purl.org/dc/terms/identifier"doi:10.1111/j.1600-0897.1998.tb00408.x"xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/author"Joly E."xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/author"Fernandez N."xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/author"Cooper J.C."xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/author"Dealtry G.B."xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/name"Am J Reprod Immunol"xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/pages"165-171"xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/title"Regulation of major histocompatibility complex and TAP gene products in preimplantation mouse stage embryos."xsd:string
http://purl.uniprot.org/citations/9764360http://purl.uniprot.org/core/volume"40"xsd:string
http://purl.uniprot.org/citations/9764360http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9764360
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