| http://purl.uniprot.org/citations/9776180 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9776180 | http://www.w3.org/2000/01/rdf-schema#comment | "Previously, we determined that elimination of deoxycytidylate (dCMP) deaminase (DCD1) in the yeast Saccharomyces cerevisiae increases the intracellular dCTP:dTTP ratio and reduces the induction of G x C --> A x T transitions in the SUP4-o gene by ethyl methanesulfonate (EMS) and N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Simultaneously, the G x C --> C x G transversion frequency rises substantially. We attributed the first response to dCTP outcompeting dTTP for incorporation opposite O6-alkylguanine, and the second outcome to the increased dCTP pool causing error-prone repair of apurinic (AP) sites resulting from the removal or lability of N7-alkylguanine. To test the latter hypothesis, we used isogenic dcd1 strains deleted for either of two genes (MAG1: 3-methyladenine glycosylase; APN1: apurinic endonuclease) involved in the repair of N7-alkylguanine. In these backgrounds, EMS or MNNG induction of total SUP4-o mutations, G x C --> A x T transitions and G x C --> C x G transversions were reduced by >98%, >97%, and >80%, respectively. Mutation frequencies in the dcd1 apn1 strain were close to those for spontaneous mutagenesis in the wild-type parent. These findings argue that misincorporation of dCTP during repair of alkylation-induced AP sites is responsible for the increased G x C --> C x G transversion frequency in the dcd1 strain treated with EMS or MNNG. The data also demonstrate that defective repair of AP sites coupled with an elevated dCTP:dTTP ratio eliminates most EMS and MNNG mutagenesis. In addition, the results point to a role for AP sites in the production of some EMS- and MNNG-induced G x C --> A x T transitions as well as other substitutions in the dcd1 strain."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.org/dc/terms/identifier | "doi:10.1002/(sici)1098-2280(1998)32:2<173::aid-em13>3.3.co;2-d"xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "Xiao W."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "Kunz B.A."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "Scott C.A."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "Karthikeyan R."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "McQueen S.A."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "Henson E.S."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/author | "Kuschak T."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/name | "Environ Mol Mutagen"xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/pages | "173-178"xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/title | "Defects in base excision repair combined with elevated intracellular dCTP levels dramatically reduce mutation induction in yeast by ethyl methanesulfonate and N-methyl-N'-nitro-N-nitrosoguanidine."xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://purl.uniprot.org/core/volume | "32"xsd:string |
| http://purl.uniprot.org/citations/9776180 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9776180 |
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| http://purl.uniprot.org/uniprot/P22936 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9776180 |
| http://purl.uniprot.org/uniprot/P06773 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/9776180 |
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