| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Citation |
| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/2000/01/rdf-schema#comment | "Asparaginyl-tRNA (Asn-tRNA) and glutaminyl-tRNA (Gln-tRNA) are essential components of protein synthesis. They can be formed by direct acylation by asparaginyl-tRNA synthetase (AsnRS) or glutaminyl-tRNA synthetase (GlnRS). The alternative route involves transamidation of incorrectly charged tRNA. Examination of the preliminary genomic sequence of the radiation-resistant bacterium Deinococcus radiodurans suggests the presence of both direct and indirect routes of Asn-tRNA and Gln-tRNA formation. Biochemical experiments demonstrate the presence of AsnRS and GlnRS, as well as glutamyl-tRNA synthetase (GluRS), a discriminating and a nondiscriminating aspartyl-tRNA synthetase (AspRS). Moreover, both Gln-tRNA and Asn-tRNA transamidation activities are present. Surprisingly, they are catalyzed by a single enzyme encoded by three ORFs orthologous to Bacillus subtilis gatCAB. However, the transamidation route to Gln-tRNA formation is idled by the inability of the discriminating D. radiodurans GluRS to produce the required mischarged Glu-tRNAGln substrate. The presence of apparently redundant complete routes to Asn-tRNA formation, combined with the absence from the D. radiodurans genome of genes encoding tRNA-independent asparagine synthetase and the lack of this enzyme in D. radiodurans extracts, suggests that the gatCAB genes may be responsible for biosynthesis of asparagine in this asparagine prototroph."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/name | "Proc. Natl. Acad. Sci. U.S.A."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.95.22.12838"xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.org/dc/terms/identifier | "doi:10.1073/pnas.95.22.12838"xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9789001 |
| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9789001 |
| http://purl.uniprot.org/citations/9789001 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9789001 |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Min B."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Min B."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Soll D."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Soll D."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Tumbula D.L."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Tumbula D.L."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Curnow A.W."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Curnow A.W."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Pelaschier J.T."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/author | "Pelaschier J.T."xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/pages | "12838-12843"xsd:string |
| http://purl.uniprot.org/citations/9789001 | http://purl.uniprot.org/core/pages | "12838-12843"xsd:string |