| http://purl.uniprot.org/citations/9801317 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9801317 | http://www.w3.org/2000/01/rdf-schema#comment | "The roles of the bHLH-Zip protein, upstream stimulatory factor (USF), in mouse metallothionein-I (MT-I) gene expression were examined. The promoter contains a putative USF binding site which overlaps an antioxidant response element (ARE) located at -101 bp relative to the transcription start point. The USF/ARE composite element increases basal expression of the mouse MT-I gene, and partly mediates response to oxidative stress. However, other functions of this composite element and the in vivo roles for USF in MT-I promoter functions have not been examined. We report studies which indicate that USF participates via the USF/ARE element in cadmium responsiveness of the mouse MT-I promoter. During the course of these studies a second, higher affinity USF binding site at -223 bp was identified. Stable and transient transfection assays in mouse hepatoma cells, using the USF/ARE in the context of a minimal promoter and site-directed and truncation mutants of the MT-I promoter, revealed that the USF and the ARE sites contribute to cadmium (2-30 microM) but not zinc responsiveness, and to basal promoter activity. Overexpression of dominant-negative (dn)USF in co-transfection assays significantly attenuated cadmium induction of the USF/ARE in the context of a minimal promoter, and attenuated cadmium, but not zinc, induction of the intact MT-I promoter. A consensus E-box (CACATG) at -223 bp in the MT-I promoter was also found to bind USF in vitro , and to be constitutively footprinted in vivo . The interaction of USF with E-box1 was apparently 10-fold stronger than that with the USF/ARE. However, in contrast, E-box1 was not a strong basal promoter element nor was it metal ions responsive in mouse Hepa cells. In conclusion, these studies demonstrate a role for USF in cadmium-specific induction of the mouse MT-I gene, but bring into question an obligate role for USF in regulating basal activity of this gene. The data further suggest that USF interacts with ARE-binding proteins to influence MT-I gene expression."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.org/dc/terms/identifier | "doi:10.1093/nar/26.22.5182"xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Li Q."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Johnson J.A."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Hu N."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Andrews G.K."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Bittel D."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Chu W.A."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/author | "Daggett M.A."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/name | "Nucleic Acids Res"xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/pages | "5182-5189"xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/title | "Participation of upstream stimulator factor (USF) in cadmium-induction of the mouse metallothionein-I gene."xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://purl.uniprot.org/core/volume | "26"xsd:string |
| http://purl.uniprot.org/citations/9801317 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9801317 |
| http://purl.uniprot.org/citations/9801317 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9801317 |
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| http://purl.uniprot.org/uniprot/#_E9Q722-mappedCitation-9801317 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9801317 |
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| http://purl.uniprot.org/uniprot/#_P02802-mappedCitation-9801317 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9801317 |
| http://purl.uniprot.org/uniprot/#_Q61069-mappedCitation-9801317 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9801317 |