| http://purl.uniprot.org/citations/9822601 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9822601 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9822601 | http://www.w3.org/2000/01/rdf-schema#comment | "Binding proteins for insulin-like growth factors (IGFs) IGF-I and IGF-II, known as IGFBPs, control the distribution, function and activity of IGFs in various cell tissues and body fluids. Insulin-like growth factor-binding protein-5 (IGFBP-5) is known to modulate the stimulatory effects of IGFs and is the major IGF-binding protein in bone tissue. We have expressed two N-terminal fragments of IGFBP-5 in Escherichia coli; the first encodes the N-terminal domain of the protein (residues 1-104) and the second, mini-IGFBP-5, comprises residues Ala40 to Ile92. We show that the entire IGFBP-5 protein contains only one high-affinity binding site for IGFs, located in mini-IGFBP-5. The solution structure of mini-IGFBP-5, determined by nuclear magnetic resonance spectroscopy, discloses a rigid, globular structure that consists of a centrally located three-stranded anti-parallel beta-sheet. Its scaffold is stabilized further by two inside packed disulfide bridges. The binding to IGFs, which is in the nanomolar range, involves conserved Leu and Val residues localized in a hydrophobic patch on the surface of the IGFBP-5 protein. Remarkably, the IGF-I receptor binding assays of IGFBP-5 showed that IGFBP-5 inhibits the binding of IGFs to the IGF-I receptor, resulting in reduction of receptor stimulation and autophosphorylation. Compared with the full-length IGFBP-5, the smaller N-terminal fragments were less efficient inhibitors of the IGF-I receptor binding of IGFs."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.22.6558"xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.org/dc/terms/identifier | "doi:10.1093/emboj/17.22.6558"xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Holak T.A."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Holak T.A."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Renner C."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Renner C."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Lang K."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Lang K."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Zweckstetter M."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Zweckstetter M."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Sanchez Y."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Sanchez Y."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Demuth D."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Demuth D."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Dony C."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Dony C."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Georgescu J."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Georgescu J."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Grol M."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Grol M."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Kalus W."xsd:string |
| http://purl.uniprot.org/citations/9822601 | http://purl.uniprot.org/core/author | "Kalus W."xsd:string |