| http://purl.uniprot.org/citations/9832551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9832551 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9832551 | http://www.w3.org/2000/01/rdf-schema#comment | "We isolated two novel actin filament (F-actin)-binding proteins from rat brain and rat 3Y1 fibroblast. They were splicing variants, and we named brain big one b-nexilin and fibroblast small one s-nexilin. b-Nexilin purified from rat brain was a protein of 656 amino acids (aa) with a calculated molecular weight of 78,392, whereas s-nexilin, encoded by the cDNA isolated from rat 3Y1 cells by the reverse transcriptase-PCR method, was a protein of 606 aa with a calculated molecular weight of 71,942. b-Nexilin had two F-actin-binding domains (ABDs) at the NH2-terminal and middle regions, whereas s-nexilin had one ABD at the middle region because 64 aa residues were deleted and 14 aa residues were inserted in the first NH2-terminal ABD of b-nexilin, and thereby the first ABD lost its activity. b- and s-nexilins bound along the sides of F-actin, but only b-nexilin showed F-actin cross-linking activity. b-Nexilin was mainly expressed in brain and testis, whereas s-nexilin was mainly expressed in testis, spleen, and fibroblasts, such as rat 3Y1 and mouse Swiss 3T3 cells, but neither b-nor s-nexilin was detected in liver, kidney, or cultured epithelial cells. An immunofluorescence microscopic study revealed that s-nexilin was colocalized with vinculin, talin, and paxillin at cell-matrix adherens junction (AJ) and focal contacts, but not at cell-cell AJ, in 3Y1 cells. Overexpressed b- and s-nexilins were localized at focal contacts but not at cell-cell AJ. These results indicate that nexilin is a novel F-actin-binding protein localized at cell-matrix AJ."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.143.5.1227"xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.org/dc/terms/identifier | "doi:10.1083/jcb.143.5.1227"xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Takai Y."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Takai Y."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Nakanishi H."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Nakanishi H."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Nishioka H."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Nishioka H."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Satoh A."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Satoh A."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Ikeda W."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Ikeda W."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Ohtsuka T."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Ohtsuka T."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Momose Y."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/author | "Momose Y."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/name | "J. Cell Biol."xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/pages | "1227-1238"xsd:string |
| http://purl.uniprot.org/citations/9832551 | http://purl.uniprot.org/core/pages | "1227-1238"xsd:string |