| http://purl.uniprot.org/citations/9839460 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9839460 | http://www.w3.org/2000/01/rdf-schema#comment | "In situ Fourier transform infrared microspectroscopy was used to study the heat stability of proteins and hydrogen bonding interactions in dry maturation-defective mutant seeds of Arabidopsis thaliana. alpha-Helical, turn and beta-sheet conformations were the major protein secondary structures in all of these seeds. On heating, intermolecular extended beta-sheet structures, typical of protein denaturation, were formed in abscisic acid-insensitive (abi3) and leafy cotyledon (lec) mutant seeds. Proteins in dry wild-type seeds did not denature up to 150 degrees C, but those in dry desiccation-sensitive, lec1-1, lec1-3 and abi3-5 seeds did at 68, 89 and 87 degrees C, respectively. In the desiccation-tolerant abi3-7 and abi3-1 seeds, denaturation commenced above 120 and 135 degrees C, respectively. Seeds of the aba1-1 abi3-1 double mutant showed signs of denaturation already upon drying. The molecular packing in the seeds was studied by observing the shift in the position of the OH-stretching vibration band with temperature. The maximal rate of change of this band with temperature was much higher in the desiccation-sensitive abi3-5, aba1-1 abi3-1, lec1-1, and lec1-3 mutant seeds than in the desiccation-tolerant wild-type, abi3-1, abi3-7, and lec2-1 seeds. We interpret this to mean that the molecular packing density is higher in dry desiccation-tolerant than in dry desiccation-sensitive seeds, which is associated with a higher or lower protein denaturation temperature, respectively. The results are discussed in relation to the physiological and biochemical characteristics of these mutant seeds."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.org/dc/terms/identifier | "doi:10.1046/j.1365-313x.1998.00277.x"xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/author | "Koornneef M."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/author | "Leon-Kloosterziel K.M."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/author | "Hoekstra F.A."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/author | "Alberda M."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/author | "Wolkers W.F."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/name | "Plant J"xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/pages | "133-143"xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/title | "Properties of proteins and the glassy matrix in maturation-defective mutant seeds of Arabidopsis thaliana."xsd:string |
| http://purl.uniprot.org/citations/9839460 | http://purl.uniprot.org/core/volume | "16"xsd:string |
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