| http://purl.uniprot.org/citations/9848230 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9848230 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9848230 | http://www.w3.org/2000/01/rdf-schema#comment | "A gene encoding cytochrome P450 involved in n-alkane utilization was cloned from an n-alkane assimilating yeast, Yarrowia lipolytica CX161-1B. The RT-PCR was performed on the mRNA prepared from the cells grown on n-alkane as a template using degenerated PCR primers designed for the conserved amino acid sequences of the CYP52 family. The RT-PCR amplified fragment was then used as a probe to isolate genes coding for P450 of the CYP52 family from the genomic DNA library of the strain CX161-1B. The nucleotide sequence of one of the positive clones was determined. An open reading frame which had the same nucleotide sequence as the RT-PCR-amplified fragment was identified. It was of 523 amino acid residues, 60.2 kDa in molecular mass, and had 30-45% sequence identity with the other members of the CYP52 family of Candida species so far analysed. The expression of the P450 gene that was named as YlALK1 was induced by n-tetradecane and repressed by glycerol. A YlALK1 gene disruptant did not grow well on n-decane, but grew on longer-chain n-alkanes such as hexadecane as a sole carbon source. Introduction of YlALK1 on a plasmid to the disruptant restored the decane assimilation. These results suggest that the YlALK1 gene product is the major P450A1k to metabolize short-chain n-alkanes such as decane and dodecane in Y. lipolytica."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.org/dc/terms/identifier | "doi:10.1002/(SICI)1097-0061(199811)14:15<1387::AID-YEA333>3.3.CO;2-D"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.org/dc/terms/identifier | "doi:10.1002/(sici)1097-0061(199811)14:15<1387::aid-yea333>3.0.co;2-m"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/author | "Iida T."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/author | "Iida T."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/author | "Takagi M."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/author | "Ohta A."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/author | "Ohta A."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/name | "Yeast"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/name | "Yeast"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/pages | "1387-1397"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/pages | "1387-1397"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/title | "Cloning and characterization of an n-alkane-inducible cytochrome P450 gene essential for n-decane assimilation by Yarrowia lipolytica."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/title | "Cloning and characterization of an n-alkane-inducible cytochrome P450 gene essential for n-decane assimilation by Yarrowia lipolytica."xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://purl.uniprot.org/core/volume | "14"xsd:string |
| http://purl.uniprot.org/citations/9848230 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9848230 |
| http://purl.uniprot.org/citations/9848230 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9848230 |
| http://purl.uniprot.org/citations/9848230 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9848230 |
| http://purl.uniprot.org/citations/9848230 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9848230 |