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| http://purl.uniprot.org/citations/9865521 | http://www.w3.org/2000/01/rdf-schema#comment | "The G protein coupling of human 5-hydroxytryptamine5A (h5-ht5A) receptors was investigated in stably transfected human embryonic kidney (HEK) 293 cells, using radioligand and guanosine-5'[gamma-35S]thiotriphosphate binding to membranes and cyclic adenosine monophosphate measurements in cells. 5-Carboxamido[3H]tryptamine bound to high- and low-affinity sites on h5-ht5A-HEK 293 cell membranes. Guanylyl-imidodiphosphate addition and pertussis toxin pre-treatment abolished high-affinity binding, indicating coupling to G proteins of the Gi/Go family. [N-methyl-3H]Lysergic acid diethylamide bound to a single site; guanylyl-imidodiphosphate and pertussis toxin did not alter lysergic acid diethylamide affinity. 5-Hydroxytryptamine stimulated guanosine-5'[gamma-35S]thiotriphosphate binding to 130% over basal and this effect was completely abolished by pertussis toxin. Various 5-hydroxytryptamine receptor ligands were tested for inhibition of 5-carboxamido[3H]tryptamine binding and in guanosine-5'[gamma-35S]thiotriphosphate binding assays. 5-Hydroxytryptamine consistently inhibited forskolin-induced cyclic adenosine monophosphate formation by 25% in h5-ht5A-HEK 293 cells; no effect was detected on basal cyclic adenosine monophosphate levels, on intracellular Ca2+ concentration or arachidonic acid release. Our studies demonstrate functional coupling of the h5-ht5A receptor to pertussis toxin-sensitive G proteins and to inhibition of adenylate cyclase activity."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.org/dc/terms/identifier | "doi:10.1016/s0014-2999(98)00744-4"xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/author | "Jurzak M."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/author | "Luyten W.H."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/author | "Leysen J.E."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/author | "Francken B.J."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/author | "Vanhauwe J.F."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/date | "1998"xsd:gYear |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/name | "Eur J Pharmacol"xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/pages | "299-309"xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/title | "The human 5-ht5A receptor couples to Gi/Go proteins and inhibits adenylate cyclase in HEK 293 cells."xsd:string |
| http://purl.uniprot.org/citations/9865521 | http://purl.uniprot.org/core/volume | "361"xsd:string |
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