| http://purl.uniprot.org/citations/9882681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9882681 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9882681 | http://www.w3.org/2000/01/rdf-schema#comment | "Sequencing of a 4.3-kb DNA region from the chromosome of Streptomyces argillaceus, a mithramycin producer, revealed the presence of two open reading frames (ORFs). The first one (orfA) codes for a protein that resembles several transport proteins. The second one (mtmR) codes for a protein similar to positive regulators involved in antibiotic biosynthesis (DnrI, SnoA, ActII-orf4, CcaR, and RedD) belonging to the Streptomyces antibiotic regulatory protein (SARP) family. Both ORFs are separated by a 1.9-kb, apparently noncoding region. Replacement of the mtmR region by an antibiotic resistance cassette completely abolished mithramycin biosynthesis. Expression of mtmR in a high-copy-number vector in S. argillaceus caused a 16-fold increase in mithramycin production. The mtmR gene restored actinorhodin production in Streptomyces coelicolor JF1 mutant, in which the actinorhodin-specific activator ActII-orf4 is inactive, and also stimulated actinorhodin production by Streptomyces lividans TK21. A 241-bp region located 1.9 kb upstream of mtmR was found to be repeated approximately 50 kb downstream of mtmR at the other end of the mithramycin gene cluster. A model to explain a possible route for the acquisition of the mithramycin gene cluster by S. argillaceus is proposed."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.181.2.642-647.1999"xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Mendez C."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Mendez C."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Salas J.A."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Salas J.A."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Brana A.F."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Brana A.F."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Lombo F."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/author | "Lombo F."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/name | "J Bacteriol"xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/pages | "642-647"xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/pages | "642-647"xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/title | "The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/title | "The mithramycin gene cluster of Streptomyces argillaceus contains a positive regulatory gene and two repeated DNA sequences that are located at both ends of the cluster."xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/volume | "181"xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://purl.uniprot.org/core/volume | "181"xsd:string |
| http://purl.uniprot.org/citations/9882681 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9882681 |
| http://purl.uniprot.org/citations/9882681 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9882681 |
| http://purl.uniprot.org/citations/9882681 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9882681 |