| http://purl.uniprot.org/citations/9885218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9885218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9885218 | http://www.w3.org/2000/01/rdf-schema#comment | "Several studies suggest membrane trafficking events are mediated by integral, membrane proteins from both transport-vesicle and target membranes, called v- and t-SNAREs (SNAp REceptors), respectively. Previous experiments using antibodies to synaptobrevin/vesicle associated membrane protein (VAMP) 1, 2, or rat cellubrevin failed to detect these v-SNAREs in human platelets, although membrane proteins from these cells could support 20S complex formation. To identify v-SNAREs in platelets, we used a polymerase chain reaction (PCR) approach with degenerate primers to amplify potential VAMP-like v-SNAREs. A cDNA encoding a novel v-SNARE was isolated from a human megakaryocyte cDNA library. Termed human cellubrevin (Hceb), this protein has greater than 93% identity with human VAMP 1, 2, and rat cellubrevin over the conserved core region, but has a unique N-terminal domain. Northern blot analysis showed that the 2. 5-kB mRNA encoding Hceb is expressed in every human tissue tested. Hceb from detergent-solubilized platelet membranes, participated in alpha-SNAP-dependent 20S complex formation and adenosine triphosphate (ATP)-dependent disassembly, showing that Hceb can act as a v-SNARE in platelets. Immunofluorescence microscopy, using an anti-Hceb antibody showed a punctate, intracellular staining pattern in platelets, megakaryocytes, and HEK-293 cells. This same pattern was observed in surface-activated platelets even though all dense core and most alpha-granule contents had been released. These data suggest that Hceb may reside on a platelet organelle that is not primarily involved in the exocytic pathway."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/author | "Whiteheart S.W."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/author | "Whiteheart S.W."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/author | "Bernstein A.M."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/author | "Bernstein A.M."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/name | "Blood"xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/pages | "571-579"xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/pages | "571-579"xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/title | "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/title | "Identification of a cellubrevin/vesicle associated membrane protein 3 homologue in human platelets."xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/volume | "93"xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://purl.uniprot.org/core/volume | "93"xsd:string |
| http://purl.uniprot.org/citations/9885218 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9885218 |
| http://purl.uniprot.org/citations/9885218 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/9885218 |
| http://purl.uniprot.org/citations/9885218 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9885218 |
| http://purl.uniprot.org/citations/9885218 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/9885218 |
| http://purl.uniprot.org/uniprot/Q15836 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/9885218 |
| http://purl.uniprot.org/uniprot/Q15836#attribution-DB96B05B7E6CEB30D8988576CA112EB5 | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/9885218 |
| http://purl.uniprot.org/uniprot/Q15836#attribution-E5641270012EABDF31FCBB641AF90C8D | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/9885218 |
| http://purl.uniprot.org/uniprot/#_Q15836-citation-9885218 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/9885218 |