| http://purl.uniprot.org/citations/9915832 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9915832 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9915832 | http://www.w3.org/2000/01/rdf-schema#comment | "Using a cytoplasmic domain of the peripheral benzodiazepine receptor (PBR) as a bait in the yeast two-hybrid system, we have isolated a cDNA encoding a new protein that specifically interacts with PBR. We named it PRAX-1, for peripheral benzodiazepine receptor-associated protein 1. PRAX-1 is a 1857-amino acid protein, the sequence of which was structurally unrelated to any known proteins. The gene encoding PRAX-1 is located in the q22-q23 region of the long arm of the human chromosome 17. The PRAX-1 mRNA is 7.5 kilobase pairs, predominantly expressed in the central nervous system, pituitary gland, and thymus. At the protein level, we found the PRAX-1 as a single 220-250-kDa protein in the brain and in many different human cell lines tested using specific antibody raised against PRAX-1. Parallel analysis of the PRAX-1 mRNA and protein expression performed in mouse and rat gave similar results. Immunocytochemistry analysis carried out to define the distribution of the PRAX-1 protein in the rat brain showed that PRAX-1 was prevalent in the mesolimbic system, specially abundant in the CA1 subfield of the hippocampus. Exhibiting several domains involved in protein-protein interaction (three proline-rich domains, three leucine-zipper motifs, and an Src homology region 3-like domain), the PRAX-1 may be looked upon as a new adaptator protein. We show that both the Src homology region 3-like domain and a proline-rich domain in PRAX-1 are required for the interaction with PBR. PRAX-1 is a cytoplasmic protein that also partially colocalizes with PBR in the mitochondria, as determined by confocal microscopy and Western blotting. Altogether our observations support a model of interaction implicating PBR and this newly described protein, PRAX-1. As being the first cytoplasmic protein associated with PBR, PRAX-1 is a new tool that opens new fields for exploring PBR biological roles."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.5.2938"xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.274.5.2938"xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Jbilo O."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Jbilo O."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Le Fur G."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Le Fur G."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Carayon P."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Carayon P."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Casellas P."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Casellas P."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Galiegue S."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Galiegue S."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Combes T."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Combes T."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Bribes E."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/author | "Bribes E."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/pages | "2938-2952"xsd:string |
| http://purl.uniprot.org/citations/9915832 | http://purl.uniprot.org/core/pages | "2938-2952"xsd:string |