| http://purl.uniprot.org/citations/9952443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9952443 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/9952443 | http://www.w3.org/2000/01/rdf-schema#comment | "In maize (Zea mays L.) two leaf-specific ferredoxin (Fd) isoproteins, Fd I and Fd II, are distributed differentially in mesophyll and bundle-sheath cells. A novel cDNA encoding the precursor of Fd II (pFD2) was isolated by heterologous hybridization using a cDNA for Fd I (pFD1) as a probe. The assignment of the cDNAs to the Fds was verified by capillary liquid-chromatography/electrospray ionization-mass spectrometry. RNA-blot analysis demonstrated that transcripts for Fd I and Fd II accumulated specifically in mesophyll and bundle-sheath cells, respectively. The mature regions of pFD1 and pFD2 were expressed in Escherichia coli as functional Fds. Fd I and Fd II had similar redox potentials of -423 and -406 mV, respectively, but the Km value of Fd-NADP+ reductase for Fd II was about 3-fold larger than that for Fd I. Asparagine at position 65 of Fd II is a unique residue compared with Fd I and other Fds from various plants, which have aspartic acid or glutamic acid at the corresponding position as an electrostatic interaction site with Fd-NADP+ reductase. Substitution of asparagine-65 with aspartic acid increased the affinity of Fd II with Fd-NADP+ reductase to a level comparable to that of Fd I. These structural and functional differences of Fd I and Fd II may be related to their cell-specific expression in the leaves of a C4 plant."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.119.2.481"xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.119.2.481"xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Hase T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Hase T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Sakakibara H."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Sakakibara H."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Sugiyama T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Sugiyama T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Kimata-Ariga Y."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Kimata-Ariga Y."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Takao T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Takao T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Shimonishi Y."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Shimonishi Y."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Murata H."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Murata H."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Matsumura T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/author | "Matsumura T."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/date | "1999"xsd:gYear |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |
| http://purl.uniprot.org/citations/9952443 | http://purl.uniprot.org/core/name | "Plant Physiol."xsd:string |