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http://purl.uniprot.org/citations/9990507http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9990507http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/9990507http://www.w3.org/2000/01/rdf-schema#comment"

Background

In the ubiquitin-dependent proteolysis pathway, a ubiquitin ligase (E3) is responsible for substrate selectivity and timing of degradation. A novel E3, SCF (Skp1-Cullin-1/Cdc53-F-box) plays a pivotal role in cell cycle progression. In fission yeast, F-box/WD-repeat protein Pop1 regulates the level of the CDK (cyclin-dependent kinase) inhibitor Rum1 and the S phase regulator Cdc18.

Results

We have cloned and characterized the pop2+ gene which encodes the Pop1-related F-box/WD-repeat protein. Pop2 plays a role which overlaps with Pop1 in the degradation of Rum1 and Cdc18. However, these two proteins are not functional homologues. Pop1 and Pop2 form hetero-as well as homo-dimers in the cell. We have analysed two fission yeast cullin members and found that cullin-1 functions as a component of SCFPop1,2, whilst cullin-3 is involved in the distinct stress-response pathway.

Conclusions

Fission yeast SCF is composed of Pop1 and Pop2, two structurally related but functionally independent F-box/WD-repeat proteins. By forming three distinct complexes, SCFPop1/Pop1, SCFPop1/Pop2 and SCFPop2/Pop2, SCF has evolved a sophisticated mechanism to control the level of Rum1 and Cdc18. Fission yeast SCF also contains cullin-1 as a universal scaffold and each cullin member plays a distinct biological role."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.1998.00225.x"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.org/dc/terms/identifier"doi:10.1046/j.1365-2443.1998.00225.x"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/author"Toda T."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/author"Toda T."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/author"Kominami K."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/author"Kominami K."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/author"Ochotorena I."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/author"Ochotorena I."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/date"1998"xsd:gYear
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/name"Genes Cells"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/pages"721-735"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/pages"721-735"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/title"Two F-box/WD-repeat proteins Pop1 and Pop2 form hetero- and homo-complexes together with cullin-1 in fission yeast SCF (Skip-cullin-1-F-box) ubiquitin ligase."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/title"Two F-box/WD-repeat proteins Pop1 and Pop2 form hetero- and homo-complexes together with cullin-1 in fission yeast SCF (Skip-cullin-1-F-box) ubiquitin ligase."xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/volume"3"xsd:string
http://purl.uniprot.org/citations/9990507http://purl.uniprot.org/core/volume"3"xsd:string
http://purl.uniprot.org/citations/9990507http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9990507
http://purl.uniprot.org/citations/9990507http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/9990507
http://purl.uniprot.org/citations/9990507http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/9990507
http://purl.uniprot.org/citations/9990507http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/9990507
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