| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Proteome |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Reference_Proteome |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/2000/01/rdf-schema#comment | "Escherichia coli B serves as a research model for studying phage sensitivity, restriction-modification systems, and bacterial evolution, and also as a workhorse for protein expression in life science laboratories and in the biotech industry. Characteristics such as protease deficiency, low acetate production at a high level of glucose, and enhanced permeability (probably due to a simple cell surface) make E. coli B a desirable host for the production of genetically engineered proteins. BL21(DE3) has been very widely used to express recombinant proteins. Comparison of BL21(DE3) and another B strain REL606 (ECOBR) shows they differ in length by 72,304 bp and have 426 single nucleotide polymorphisms, all of which have been accounted for by various laboratory manipulations. Differences between B strains and K12 include the absence of flagellar component genes, the DNA cytosine methylase dcm, and ompT in BL21(DE3). B strains may have an additional type II secretion system not found in K12. Other differences include differential distribution of insertions sequences and other changes resulting from horizontal gene transfer. BL21(DE3) also carries a DE3 recombinant phage harboring the T7 RNA polymerase gene that can direct high-level expression of cloned genes under the control of the T7 promoter (adapted from PMID 19786035)."xsd:string |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/2000/01/rdf-schema#comment | "Escherichia coli is a Gram-negative straight rod, which either uses peritrichous flagella for mobility or is nonmotile. It is a facultatively anaerobic chemoorganotroph capable of both respiratory and fermentative metabolism. E.coli serves a useful function in the body by suppressing the growth of harmful bacterial species and by synthesising appreciable amounts of vitamins. It is an important component of the biosphere. It colonizes the lower gut of animals and survives when released to the natural environment, allowing widespread dissemination to new hosts. Pathogenic E.coli strains are responsible for infection of the enteric, urinary, pulmonary and nervous systems. Comparison of 20 E.coli/Shigella strains shows the core genome to be about 2000 genes while the pan-genome has over 18,000 genes. There are multiple, striking integration hotspots that are conserved across the genomes, corresponding to regions of abundant and parallel insertions and deletions of genetic material."xsd:string |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/2000/01/rdf-schema#seeAlso | http://purl.uniprot.org/proteomes/UP000002032#assembly |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/2000/01/rdf-schema#seeAlso | http://purl.uniprot.org/proteomes/UP000002032#source |
| http://purl.uniprot.org/proteomes/UP000002032 | http://purl.uniprot.org/core/organism | http://purl.uniprot.org/taxonomy/469008 |
| http://purl.uniprot.org/proteomes/UP000002032 | http://purl.uniprot.org/core/citation | http://purl.uniprot.org/citations/SIP48E94024B54FAA41 |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/2004/02/skos/core#closeMatch | http://purl.uniprot.org/proteomes/UP000002032#cpd |
| http://purl.uniprot.org/proteomes/UP000002032 | http://purl.org/dc/terms/modified | "2023-11-20"xsd:date |
| http://purl.uniprot.org/proteomes/UP000002032 | http://www.w3.org/2004/02/skos/core#narrower | http://purl.uniprot.org/proteomes/UP000002032#Chromosome |
| http://purl.uniprot.org/proteomes/UP000002032 | http://busco.ezlab.org/schema#has_score | http://purl.uniprot.org/proteomes/UP000002032#busco |
| http://purl.uniprot.org/proteomes/UP000002032 | http://purl.uniprot.org/core/panproteome | http://purl.uniprot.org/proteomes/UP000000625 |
| http://purl.uniprot.org/proteomes/UP000002032 | http://purl.uniprot.org/core/strain | http://purl.uniprot.org/proteomes/UP000002032#B%20%2F%20BL21-DE3%20%5BJGI%5D |
| http://purl.uniprot.org/uniprot/A0A140N8M7#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N7I0#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N662#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N6F9#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N7U2#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140NC39#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140NGD0#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N7Z4#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N7R9#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140N644#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |
| http://purl.uniprot.org/uniprot/A0A140NA90#attribution-3281418D0D255DEC6B4AAA30778772FF | http://purl.uniprot.org/core/source | http://purl.uniprot.org/proteomes/UP000002032 |