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Entry version 180 (31 Jul 2019)
Sequence version 2 (05 Oct 2010)
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Protein

Eukaryotic peptide chain release factor subunit 1

Gene

SUP45

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the ‘correct annotation’ for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>
-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the ‘protein existence’ evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Directs the termination of nascent peptide synthesis (translation) in response to the termination codons UAA, UAG and UGA.By similarity1 Publication

Miscellaneous

Present with 13100 molecules/cell in log phase SD medium.1 Publication

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Biological processProtein biosynthesis

Enzyme and pathway databases

BioCyc Collection of Pathway/Genome Databases

More...
BioCyci
YEAST:G3O-29097-MONOMER

Reactome - a knowledgebase of biological pathways and processes

More...
Reactomei
R-SCE-72764 Eukaryotic Translation Termination
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Eukaryotic peptide chain release factor subunit 1
Short name:
Eukaryotic release factor 1
Short name:
eRF1
Alternative name(s):
Omnipotent suppressor protein 1
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: ‘Name’, ‘Synonyms’, ‘Ordered locus names’ and ‘ORF names’.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:SUP45
Synonyms:SAL4, SUP1
Ordered Locus Names:YBR143C
ORF Names:YBR1120
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the ‘taxonomic identifier’ or ‘taxid’.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri559292 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
<p>This subsection of the <a href="http://www.uniprot.org/help/names_and_taxonomy_section">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000002311 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes_manual">proteome</a> can consist of several components. <br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome II

Organism-specific databases

Eukaryotic Pathogen Database Resources

More...
EuPathDBi
FungiDB:YBR143C

Saccharomyces Genome Database

More...
SGDi
S000000347 SUP45

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section describes the extent of a polypeptide chain in the mature protein following processing.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00001431671 – 437Eukaryotic peptide chain release factor subunit 1Add BLAST437

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘PTM / Processing’ section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei182N5-methylglutamine2 Publications1
<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM / Processing</a> section describes <strong>covalent linkages</strong> of various types formed <strong>between two proteins (interchain cross-links)</strong> or <strong>between two parts of the same protein (intrachain cross-links)</strong>, except the disulfide bonds that are annotated in the <a href="http://www.uniprot.org/manual/disulfid">'Disulfide bond'</a> subsection.<p><a href='/help/crosslnk' target='_top'>More...</a></p>Cross-linki331Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources
Modified residuei421PhosphoserineCombined sources1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm_processing_section">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

N5-methylated on Gln-182 by MTQ2.2 Publications

Keywords - PTMi

Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase

More...
MaxQBi
P12385

PaxDb, a database of protein abundance averages across all three domains of life

More...
PaxDbi
P12385

PRoteomics IDEntifications database

More...
PRIDEi
P12385

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...
iPTMneti
P12385

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction_section">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Heterodimer of two subunits, one of which binds GTP.

Interacts with TPA1.

1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction_section%27">Interaction</a> section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="http://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated on a monthly basis. Each binary interaction is displayed on a separate line.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGrid)

More...
BioGridi
32843, 122 interactors

ComplexPortal: manually curated resource of macromolecular complexes

More...
ComplexPortali
CPX-435 Translation release factor ERF1-ERF3 complex

Database of interacting proteins

More...
DIPi
DIP-800N

Protein interaction database and analysis system

More...
IntActi
P12385, 46 interactors

Molecular INTeraction database

More...
MINTi
P12385

STRING: functional protein association networks

More...
STRINGi
4932.YBR143C

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...
SMRi
P12385

Database of comparative protein structure models

More...
ModBasei
Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the eukaryotic release factor 1 family.Curated

Phylogenomic databases

Ensembl GeneTree

More...
GeneTreei
ENSGT00390000009004

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...
HOGENOMi
HOG000224681

InParanoid: Eukaryotic Ortholog Groups

More...
InParanoidi
P12385

KEGG Orthology (KO)

More...
KOi
K03265

Identification of Orthologs from Complete Genome Data

More...
OMAi
GPGTEKM

Family and domain databases

Gene3D Structural and Functional Annotation of Protein Families

More...
Gene3Di
3.30.1330.30, 1 hit
3.30.420.60, 1 hit
3.30.960.10, 1 hit

Integrated resource of protein families, domains and functional sites

More...
InterProi
View protein in InterPro
IPR042226 eFR1_2_sf
IPR005140 eRF1_1_Pelota
IPR005141 eRF1_2
IPR005142 eRF1_3
IPR029064 L30e-like
IPR004403 Peptide_chain-rel_eRF1/aRF1
IPR024049 Release_factor_eRF1/aRF1_N

The PANTHER Classification System

More...
PANTHERi
PTHR10113 PTHR10113, 1 hit

Pfam protein domain database

More...
Pfami
View protein in Pfam
PF03463 eRF1_1, 1 hit
PF03464 eRF1_2, 1 hit
PF03465 eRF1_3, 1 hit

Simple Modular Architecture Research Tool; a protein domain database

More...
SMARTi
View protein in SMART
SM01194 eRF1_1, 1 hit

Superfamily database of structural and functional annotation

More...
SUPFAMi
SSF55315 SSF55315, 1 hit
SSF55481 SSF55481, 1 hit

TIGRFAMs; a protein family database

More...
TIGRFAMsi
TIGR03676 aRF1/eRF1, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence_length">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequencei

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences_section">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

P12385-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDNEVEKNIE IWKVKKLVQS LEKARGNGTS MISLVIPPKG QIPLYQKMLT
60 70 80 90 100
DEYGTASNIK SRVNRLSVLS AITSTQQKLK LYNTLPKNGL VLYCGDIITE
110 120 130 140 150
DGKEKKVTFD IEPYKPINTS LYLCDNKFHT EVLSELLQAD DKFGFIVMDG
160 170 180 190 200
QGTLFGSVSG NTRTVLHKFT VDLPKKHGRG GQSALRFARL REEKRHNYVR
210 220 230 240 250
KVAEVAVQNF ITNDKVNVKG LILAGSADFK TDLAKSELFD PRLACKVISI
260 270 280 290 300
VDVSYGGENG FNQAIELSAE ALANVKYVQE KKLLEAYFDE ISQDTGKFCY
310 320 330 340 350
GIDDTLKALD LGAVEKLIVF ENLETIRYTF KDAEDNEVIK FAEPEAKDKS
360 370 380 390 400
FAIDKATGQE MDVVSEEPLI EWLAANYKNF GATLEFITDK SSEGAQFVTG
410 420 430
FGGIGAMLRY KVNFEQLVDE SEDEYYDEDE GSDYDFI
Length:437
Mass (Da):49,006
Last modified:October 5, 2010 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i9B59133EE3B1F61E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the ‘Sequence’ section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti41Q → L in CAA27719 (PubMed:3526282).Curated1
Sequence conflicti41Q → L in M28042 (PubMed:3037308).Curated1

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...
EMBLi

GenBank nucleotide sequence database

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GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...
DDBJi
Links Updated
X04082 Genomic DNA Translation: CAA27719.1
M28042 Genomic DNA No translation available.
Z36012 Genomic DNA Translation: CAA85101.1
X73531 Genomic DNA Translation: CAA51935.1
BK006936 Genomic DNA Translation: DAA07259.1

Protein sequence database of the Protein Information Resource

More...
PIRi
S46014

NCBI Reference Sequences

More...
RefSeqi
NP_009701.3, NM_001178491.3

Genome annotation databases

Ensembl fungal genome annotation project

More...
EnsemblFungii
YBR143C_mRNA; YBR143C_mRNA; YBR143C

Database of genes from NCBI RefSeq genomes

More...
GeneIDi
852440

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...
KEGGi
sce:YBR143C

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X04082 Genomic DNA Translation: CAA27719.1
M28042 Genomic DNA No translation available.
Z36012 Genomic DNA Translation: CAA85101.1
X73531 Genomic DNA Translation: CAA51935.1
BK006936 Genomic DNA Translation: DAA07259.1
PIRiS46014
RefSeqiNP_009701.3, NM_001178491.3

3D structure databases

Select the link destinations:

Protein Data Bank Europe

More...
PDBei

Protein Data Bank RCSB

More...
RCSB PDBi

Protein Data Bank Japan

More...
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4CRMelectron microscopy8.75X140-421[»]
4CRNelectron microscopy9.10X1-437[»]
SMRiP12385
ModBaseiSearch...

Protein-protein interaction databases

BioGridi32843, 122 interactors
ComplexPortaliCPX-435 Translation release factor ERF1-ERF3 complex
DIPiDIP-800N
IntActiP12385, 46 interactors
MINTiP12385
STRINGi4932.YBR143C

PTM databases

iPTMnetiP12385

Proteomic databases

MaxQBiP12385
PaxDbiP12385
PRIDEiP12385

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYBR143C_mRNA; YBR143C_mRNA; YBR143C
GeneIDi852440
KEGGisce:YBR143C

Organism-specific databases

EuPathDBiFungiDB:YBR143C
SGDiS000000347 SUP45

Phylogenomic databases

GeneTreeiENSGT00390000009004
HOGENOMiHOG000224681
InParanoidiP12385
KOiK03265
OMAiGPGTEKM

Enzyme and pathway databases

BioCyciYEAST:G3O-29097-MONOMER
ReactomeiR-SCE-72764 Eukaryotic Translation Termination
R-SCE-975956 Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-SCE-975957 Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

Protein Ontology

More...
PROi
PR:P12385

Family and domain databases

Gene3Di3.30.1330.30, 1 hit
3.30.420.60, 1 hit
3.30.960.10, 1 hit
InterProiView protein in InterPro
IPR042226 eFR1_2_sf
IPR005140 eRF1_1_Pelota
IPR005141 eRF1_2
IPR005142 eRF1_3
IPR029064 L30e-like
IPR004403 Peptide_chain-rel_eRF1/aRF1
IPR024049 Release_factor_eRF1/aRF1_N
PANTHERiPTHR10113 PTHR10113, 1 hit
PfamiView protein in Pfam
PF03463 eRF1_1, 1 hit
PF03464 eRF1_2, 1 hit
PF03465 eRF1_3, 1 hit
SMARTiView protein in SMART
SM01194 eRF1_1, 1 hit
SUPFAMiSSF55315 SSF55315, 1 hit
SSF55481 SSF55481, 1 hit
TIGRFAMsiTIGR03676 aRF1/eRF1, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...
ProtoNeti
Search...

MobiDB: a database of protein disorder and mobility annotations

More...
MobiDBi
Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the ‘Entry information’ section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiERF1_YEAST
<p>This subsection of the ‘Entry information’ section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called ‘Primary (citable) accession number’.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: P12385
Secondary accession number(s): D6VQD9
<p>This subsection of the ‘Entry information’ section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification (‘Last modified’). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical_and_isoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1989
Last sequence update: October 5, 2010
Last modified: July 31, 2019
This is version 180 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the ‘Entry information’ section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names
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